Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae

Industrial Crops and Products, Volume 63, January 2015, Pages 175-181

Zuo-Fu Wei, Xi-Qing Wang, Xiao Peng, Wei Wang, Chun-Jian Zhao, Yuan-Gang Zu, Yu-Jie Fu

Abstract

In this study, fast and green extraction and separation of four main flavonoids in Radix Scutellariae wasachieved by natural deep eutectic solvent microwave-assisted extraction (NADES-MAE) and direct mac-roporous resin adsorption and desorption process. Multivariate data analysis revealed that the NADESsystem composed of choline chloride and lactic acid, possessed excellent extractability for baicalin,wogonoside, baicalein and wogonin. The influential parameters of NADES-MAE were investigated andoptimized by single-factor experiment and Box–Behnken experimental design combined with responsesurface methodology. Well-fitting models for the extraction of baicalin, wogonoside, baicalein and wogo-nin were successfully established and verified. The proposed method had satisfactory extraction yields forthe four flavonoids and high reproducibility. Moreover, the direct enrichment and separation of baicalin,wogonoside, baicalein and wogonin in NADES extraction solution was efficiently achieved using mac-roporous resin ME-2 with recovery yields of 84.1%, 79.5%, 85.0% and 81.8%, respectively. The developedprocedure represents an excellent alternative for the extraction and separation of natural products fromsample matrices.